• High level of activation • Coupled product is very stable, especially at high pH. NHS-activated Sepharose 4 Fast Flow is a bead-formed, highly cross-linked pre-activated matrix. NHS-activated Sepharose 4 Fast Flow has a high level of activity and gives a very stable coupled product. Shop online for a wide selection of GE Healthcare NHS Mag Sepharose NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample.

Analysis Note To view the Certificate of Analysis for this product, please visit. Features and Benefits • For coupling ligands via primary amines. • Simple coupling, carried out at close to neutral pH. • Delivered in 100% isopropanol to protect the active groups.

• 10 μmol NHS groups/mL medium. General description HiTrap ® NHS activated HP columns are prepacked with NHS activated Sepharose ® High Performance. This high-performance medium is designed for easy coupling of ligands containing primary amines. HiTrap ® NHS-activated HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTAdesign or FPLC ™ System. Legal Information FPLC is a trademark of GE Healthcare HiTrap is a registered trademark of GE Healthcare Sepharose is a registered trademark of GE Healthcare.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer. Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations.

The NHS-activated magnetic product consists of super-paramagnetic beads which are functionalized with amino-reactive groups. For covalent coupling of molecules with primary amino ­groups such as peptides, proteins, antibodies and aptamers. Subsequently, target proteins are affinity purified using magnetic separation technology. The magnetic beads show superior magnetic behavior and are easily attracted to external magnets allowing separation within seconds.

The matrix enables minimal nonspecific binding of proteins etc. Due to its hydrophilic nature. The beads are black and easily observed by the eye and dense making them easy to spot and collect. The beads do not aggregate and are easily re-suspended in most buffers. The coupling capacity is general 1-10 mg protein per ml beads. The quantity of beads needed can easily be scaled up or down to match antibody concentration and sample volumes. The beads are suitable for separations from ul to 500 ml scale using appropriate magnetic separators.

Description: Protein Coupling • There are 30mg Magnetic beads in the centrifuge tube. • Dissolve 0.5-1mg protein/peptide in 1ml coupling buffer. Note: Coupling efficiencies to NHS-activated magnetic beads vary from ligand to ligand. The user should empirically optimize the concentration of the ligand. 0.5-10 mg/ml is recommended for protein conjugation.

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For small peptides, the concentration of ligand should be at least 200 um ligand per ml. • Add the protein solution to the beads. Resuspend the magnetic beads and mix very well by pipetting or vortexing.

Incubate the reaction with continuous rotation at room temperature for 4-6 hours or overnight. • Place the tube on the magnetic separator for 1-3minutes. Remove the supernatant while the tube remains on the separator. Remove the tube from the separator and resuspend the beads with 1 ml wash buffer by vortex for 30 seconds. Place the tube on the magnetic separator for 1-3 minutes. Remove the supernatant while the tube remains on the separator.